Representative image of SYTO®13 (green)/EB (red) fluorescence used for membrane integrity assay. Green cells have intact membranes and red cells have damaged membranes. Picture from Prickett et al., 2015.
November's Paper of the Month is now live!
It comes from a team at the University of Alberta, who have been studying how intracellular ice formation during cryopreservation of cells is affected by the degree of supercooling and the cell volume.
In this study the number of human umbilical vein endothelial cells (HUVECs) undergoing intracellular ice formation at different degrees of supercooling were examined on a cryostage. Intracellular freezing can be detected by the darkening of cells. Cell survival after thawing was determined using a membrane integrity assay. In the picture above, the cells with intact membranes can be seen in green and the cells with damaged membranes can be seen in red.