CLEM Co-ordinate Conversion Tool

 

Our CMS196 stages have an integrated, encoded, XY mechanism making it easy to accurately identify the coordinates of points of interest (POIs) on the EM grid. Using the NEXUS software and imaging system, the coordinates of POIs can also be identified on the high resolution map image created by NEXUS software. However it can be difficult to translate the co-ordinate system of the fluorescence microscope to that of the EM.

Linkam now provide a simple tool to quickly and easily transform the CMS196 co-ordinates from the fluorescence map to the EM co-ordinate system

CLEM Coordinator: coordinate translation tool. (c) Linkam Scientific

The CLEM coordinate conversion tool below is platform-independent. The coordinates of a point of interest from the fluorescence map can be converted into EM coordinates as long as the coordinates for two landmarks A and B are known for the fluorescence and the EM coordinate systems. The calculation accounts for scaling, translation and rotation but not for a flip of the sample.

Landmark A, Fluorescence
 (X coord.)
 (Y coord.)
 
Landmark B, Fluorescence
 (X coord.)
 (Y coord.)
 
 
Landmark A, EM
 (X coord.)
 (Y coord.)
 
Landmark B, EM
 (X coord.)
 (Y coord.)
 
 
Point of interest, Fluorescence
 (X coord.)
 (Y coord.)
 
 
 
   
   

The calculated coordinates for your point of interest in EM (X,Y) are:

0
0