In the last two decades, cryomicroscopy methods have become widespread and effective tools used by life scientists, pharmaceutical researchers, and more, to examine biological structures close to their native state.1 The capabilities of cryomicroscopy to visualize the structure of biological molecules, such as proteins and protein complexes, make a valuable addition to existing methods such as X-ray crystallography and nuclear magnetic resonance (NMR). Determining the structure of proteins and protein complexes, which are of particular interest as drug targets and are the subject of investigation to gain a deeper understanding of disease mechanisms, is an important element of drug discovery.

在过去的二十年中，冷冻显微镜方法已经成为生命科学家、制药研究人员等广泛使用的有效工具，用于检查接近其原生状态的生物结构1。冷冻显微镜能够可视化蛋白质和蛋白质复合物等物质的生物分子结构，是对现有的方法如x射线晶体学和核磁共振(NMR)等的有价值的补充。确定蛋白质和蛋白质复合物的结构是药物发现的一个重要部分，这对研究药物靶点非常有意义，也是深入了解疾病机制的重要课题，。