Cryogenic light microscopy is an important part a correlative imaging workflow, allowing confirmation of localised molecules of interest prior to processing for downstream analysis such ultrastructural or compositional assessment or in short: It is a key mapping step.

Here, the authors describe adaptation of a standard upright widefield microscope into a cryogenic 3D laser-free confocal system using the Linkam CMS196. We demonstrate the necessary sample preparation steps followed by confocal imaging of biological cells and tissues.

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Nizamudeen, Zubair Ahmed, et al. "Adaptive 3D cryogenic correlative light imaging of native biology using laser free confocal system. Wiley Analytical Science